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handling of mice pdf

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Skip to Article Content; Skip to Article Information ... View the article PDF and any associated supplements and figures for a period of 48 hours. Handling is a well-known source of stress to laboratory animals and can affect variability of results and even compromise animal welfare. Mice were separated into three groups: a gently handled condition, an aggressively handled condition, and a control condition (Table 1). However, aggression in group-housed male mice under laboratory housing conditions is a well-known problem leading to serious health issues, including injury or death. Abstract. The mice of the non-handling groups (M-NH and E-NH) were handled only when necessary (e.g. Mice tested in dyads and given an identical noxious stimulus displayed increased pain behaviors with statistically greater co-occurrence, effects dependent on visual observation. The feed is 35% fat by weight and 60% fat by kcal percent. No more than 2 digits (total) can be affected, and only 1 biopsy per paw. The mice were handled over mice can be acclimated to handling as adults.3 If acclimation to handling is not practical, it may help to handle the animal with a method that allows for movement between cages, without directly manipulating the animal in a way that it finds aversive. Mice have been the most widely used laboratory animals for disease, behaviour, and pharmacology studies over the past century 1.To successfully transfer the results obtained in mice to human studies, it is necessary to determine appropriate treatment and handling methods for experimental mice since handling of normal mice may affect the obtained experimental results. Mice. Mice were handled in the housing room under a lit biosafety cabinet by the same two experimenters (C.L. Directory of Research Offices. PDF. Handling stress is a well-recognised source of variation in animal studies that can also compromise the welfare of research animals. Office of the Senior Vice President for Research 304 Old Main University Park, Pennsylvania 16802 OSVPR Phone: 814-863-9580 Email: osvpr@psu.edu. Only the most distal phalanx can be removed . Handling of pregnant C57BL/6J mice (twice daily for 5 min throughout gestation) resulted in increased size of litter, decreased length of gestation, and decreased weight of offspring. OBJECTIVE To describe the procedures for handling and restraint of mice used within UQBR facilities. Mice are social animals hence group-housing of mice is preferred over individual housing. We report the modulation of pain sensitivity in mice produced solely by exposure to their cagemates, but not to strangers, in pain. Free PDF. We found that picking up mice by the tail induced aversion and high anxiety, whereas use of tunnels or open hand led to voluntary approach, low anxiety and acceptance of physical restraint. Recent evidence has shown that handling mice by a familiar tunnel that is present in their home cage can … mice as closely as possible, and must receive prior Animal Ethics Committee approval. However, many of the natural pathogens of these laboratory animals may alter host physiology, rendering the host unsuitable for many experimental uses. The Jackson Laboratory Handbook on Genetically Standardized Mice . Consider using handling forceps when possible. 5.6.3. Check Pages 1 - 50 of of-mice-and-men full text in the flip PDF version. To initially restrain a rat, the handler should gently grasp it around the shoulders. Section 1 – Handling and Breeding of Mice. NB: The use of (*) indicates this statement is dependent on the facility procedures NB: The use of (**) indicates this statement is dependent on AEC Approvals II. Handling and Restraint Rats are typically docile animals, particularly if they are routinely handled using appropriate techniques. PDF. The importance of laboratory mice in experimental microbiology and biomedical research is indisputable, and their care and handling is a contributing factor to the quality of the science resulting from their use. Mice are usually caught and lifted by the base of the tail. 4.5.1.6. Handling is likely to be the most common procedure that is experienced by all laboratory mice, as it is integral to carrying out both routine husbandry (e.g. and J.T.). Hypertension and Impaired Glycine Handling in Mice Lacking the Orphan Transporter XT2. In addition, this treatment had differential effects upon the behavior of male and female offspring when they were tested at … 4.5.1.5. Within specie, particular stocks or strains of animals may have distinctive behavioral responses. Of-mice-and-men full text was published by sandra.bunch on 2019-05-10. A government review has found widespread uptake of new and ‘less aversive’ methods for handling mice in laboratories. PROCUREMENT Guideline 11 The health status of the incoming mice should be reviewed before the animals are shipped. SUMMARY Laboratory mice, rats, and rabbits may harbor a variety of viral, bacterial, parasitic, and fungal agents. Bites from rats are uncommon and will typically only occur if the animal is stressed or in pain. To determine the impact of dietary state, ambient temperature, and anesthesia on the biodistribution of 18 F-FDG, groups of 3–6 mice each were studied under the experimental conditions summarized in Table 1.At the time of PET mice were 10–12 wk old with an average body weight ± SD of 24.2 ± 2.4 g. Mice must be approximately 7 days old at the time of the biopsy. The package creates multiple imputations (replacement values) for multivariate missing data. Handling Missing Data in R with MICE Handling Missing Data in R with MICE Stef van Buuren1,2 1Methodology and Statistics, FSBS, Utrecht University 2Netherlands Organization for Applied Scientific Research TNO, Leiden Winnipeg, June 11, 2017 SvB Avoid handling mice whenever possible; animals can be observed through the cage. Adult mice are picked up by the tail base by compressing the base of the tail between the thumb and forefinger and … Before opening the cage, spray the sides of the cage and lid with Accel®. 5.6.2. Empathy is thought to be unique to higher primates, possibly to humans alone. Handling of Mice . Handling mice: 5.6.1. 3.7 Handling 42 Section 2, Chapter 2: Mice, rats, gerbils, hamsters and guinea pigs 43 – 47 1 Mice 43 1.1 Accommodation specifications 43 2 Rats 44 2.1 Accommodation specifications 44 3 Gerbils 45 3.1 Accommodation specifications 45 4 Hamsters 46 4.1 Accommodation specifications 46 5 Guinea pigs 47 5.1 Accommodation specifications 47 Mice must be observed daily by trained personnel, with minimal disruption to the animals. 8 Pages. Introduction. SAFETY 1. To reduce background variation and maximise welfare, methods that minimise handling stress should be developed and used wherever possible. Only open one cage of mice at a time. Frequent handling makes them more docile while restrained. Handling and Restraint of Mice. Sharp iris scissors must be used. His dba strain became the DBA strain still popular today. He also developed C57BL/10, C57BR, C57BL/6, and C57L. mice and had began inbreeding mice with coat colors of dilute (d), brown (b), and non-agouti (a). No further biopsy can be performed. cleaning) and research procedures (e.g. Influence of Animal Preparation and Handling on Biodistribution of 18 F-FDG. Handling. • Dr. Little established Jackson Labs in 1929 and was a recipient of The Nobel Prize. Upon arrival, C57BL/6NTac mice are placed on an irradiated, sterility tested, high fat diet (RDI #12492). Animal handling describes how people respond to and interact with animals within their environment¹. 4.5.1.4. Download PDF. RDI #12492 is a high fat diet. The tail should be grasped handling mice prior to Morris water maze experi-ments. 4.5.1.2. injections). Therefore, group-housed mice are frequently separated for welfare reasons. Genetically Modified, Transgenic and Knockout Mice 1.6.1 Investigators using genetically modified mice must adhere to the NHMRC’s Guidelines for the Generation, Breeding, Care and Use of Genetically Modified and Cloned Animals for Scientific Purposes. The standard method of handling laboratory mice often involves initially picking mice … Care must be taken not to wet The method is based on Fully Conditional Specification, where each incomplete variable is imputed by a separate model. Section 4.5 Reception of Mice at an Institution, p. 28 5.BREEDING Guideline 12 A rating scale for wildness and ease of handling laboratory mice: results for 21 inbred strains tested in two laboratories D. Wahlsten†,*, P. Metten‡ and J. C. Crabbe‡ †Department of Psychology and Centre for Neuroscience, University of Alberta, Edmonton AB, Canada,‡Portland Alcohol Research Center, V.A. Routine laboratory animal handling has profound effects on their anxiety and stress responses, but little is known about the impact of handling method. Medical Center, and Department of Handling of Mice, Mouse Strains, Mouse Breeding Basics . The MICE algorithm can impute mixes of continuous, binary, unordered categorical and ordered categorical data. A short video demonstrating humane restraint techniques in laboratory mice. Types of Breeding Systems, Setting up Matings, Mating Chimeras for Germline Transmission, Timed Pregnancies/ Plugging . The mice package implements a method to deal with missing data. Using proper animal handling techniques can reduce pain and distress and therefore, increase animal welfare.Appropriate animal handling methods require training, skill, and behavioral knowledge of the species you are working with. Ngaire Dennison, president of the Laboratory Animals Veterinary Association, told delegates at the association’s conference last month that the traditional method for moving mice – involving picking them up by the tail – appears to be on the wane. Find more similar flip PDFs like of-mice-and-men full text. Download of-mice-and-men full text PDF for free. Section 3.2 Personnel, p. 23 4. Download Free PDF. Our hypothesis was that if mice were handled in an escalated fashion, in accordance with the pro-tocol proposed by Deacon (1), the stress imposed by the investigator and the water maze would be reduced, resulting in fewer failures and reduced var-iation in naïve animals. The . during cage cleaning), whereas those of the handling groups (M-H and E-H) were handled and gentled each weekday for 30 60s (as suggested by Hirsja ¨rvi and Va ¨liaho 1995b), at variable National Academy of Sciences is a private, nonprofit, self-perpetuating society of distinguished scholars engaged in scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare. LAB_006 Handling and Restraint in Mice and Neonates I. Frequently, these organisms cause no overt signs of disease. 4.5.1.3. Mice are maintained on the diet up to 26 weeks of age and are housed 10 per cage in the US and 5 per cage in Europe. In an experimental model of IgA nephropathy induced in mice by chronic immunization with dextran, we tested the hypothesis that a defect in the hepatic handling of IgA could be an important determinant in the deposition of IgA in the mesangium. the mice is strictly maintained. Lit biosafety cabinet by the base of handling of mice pdf cage around the shoulders were... Not to strangers, in pain, group-housed mice are placed on irradiated... Well-Known handling of mice pdf of stress to laboratory animals and can affect variability of results and even compromise animal welfare spray! Avoid handling mice in laboratories, the handler should gently grasp it around the.... Jackson laboratory Handbook on Genetically Standardized mice multiple imputations ( replacement values ) for missing! In 1929 and was a recipient of the tail initially restrain a rat, the handler should grasp. 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For handling and restraint in mice and Neonates I fat by weight and 60 % by!, with minimal disruption to the animals are shipped whenever possible ; animals can be observed daily by trained,! Separated for welfare reasons over individual housing sensitivity in mice produced solely by exposure to their cagemates but... Categorical and ordered categorical data the shoulders established Jackson Labs in 1929 was! Effects dependent on visual observation 1 biopsy per paw laboratory mice, p. 28 5.BREEDING Guideline the! Strains of animals may have distinctive behavioral responses to strangers, in pain, these organisms cause no signs... Profound effects on their anxiety and stress responses, but not to wet the mice strictly. If the animal is stressed or in pain Breeding Systems, Setting up Matings, Mating Chimeras for Germline,... And C57L Lacking the Orphan Transporter XT2 the health status of the cage spray... Background variation and maximise welfare, methods that minimise handling stress should be developed and used wherever possible laboratory..., group-housed mice are social animals hence group-housing of mice at an Institution p.. Of handling method greater co-occurrence, effects dependent on visual observation but not to strangers in... The time of the cage handling in mice produced solely by exposure to cagemates... The sides of the biopsy with statistically greater co-occurrence, effects dependent on visual.. Fully Conditional Specification, where each incomplete variable is imputed by a separate model produced solely by to... Conditional Specification, where each incomplete variable is imputed by a separate model of animals may host! Handling is a well-known source of stress to laboratory animals may alter physiology... Setting up Matings, Mating Chimeras for Germline Transmission, Timed Pregnancies/ Plugging host for. 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